av MJ Yousefzadeh · 2018 · Citerat av 185 — Fisetin has anti-cancer activity and appears to block the is a topoisomerase inhibitor, which may also contribute to its anti-cancer activity [. 81.

anti-Topoisomerase I Antibody. Applications: WB, IHC, IP. Reactivity: Human (Hu) Conjugate/Tag: Unconjugated. Quantity: 50 µL.

Predicted band size DNA topoisomerase I is an enzyme responsible for the relaxation of coiled DNA. Anti-topoisomerase I antibodies (also called anti-Scl-70 antibodies) are directed against different epitopes on this enzyme (Simon et al., 2009). Ontology: Topoisomerase-1 antibody (C0700437) Derived from the NIH UMLS (Unified Medical Language System) All lanes : Anti-Topoisomerase II alpha antibody [EP1102Y] (ab52934) at 1/1000 dilution Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate Lane 2 : Jurkat (human acute T cell leukemia) whole cell lysate Lane 3 : Neuro-2a (mouse neuroblastoma) whole cell lysate Lane 4 : Human breast cancer lysate Lane 5 : Mouse thymus lysate The presence of anti-SCL-70 (anti-topoisomerase) antibodies is highly specific to the diagnosis of diffuse scleroderma, while the presence of anti-centromere antibodies is highly specific to the diagnosis of limited scleroderma. The presence of Scl-70 antibodies (also referred to as topoisomerase I, topo-I or ATA) is considered diagnostic for systemic sclerosis (SSc). Scl-70 antibodies alone are detected in about 20 percent of SSc patients and are associated with the diffuse form of the disease, which may include specific organ involvement and poor prognosis. 24 antibodies to Topoisomerase I and validated for use in 7 applications (Immunohistochemistry, Western Blot, Immunoprecipitation, Flow Cytometry, ChIP assay, Immunocytochemistry, Immunofluorescence) There was a significantly positive association between anti-topoisomerase I Ab levels and MRSS and a significantly negative association with disease duration by multiple regression analysis. Our findings suggest that serum levels of anti-topoisomerase I Ab reflect the severity of skin sclerosis in patients with SSc. Anti-topoisomerase antibodies can be classified according to their immunoglobulin class (IgM, IgG or IgA). IgG-ATA is found most frequently in scleroderma, with IgA being quite common but IgM very infrequent.

Scl70) are serological markers of diffuse systemic sclerosis. (1) and among its fundamental diagnostic- Apr 18, 2018 reporting clinical significance of human autoantibody to DNA topoisomerase I ( anti-topo I) in patients with non-small cell lung cancer (NSCLC). Fast, simple, high performance LANCE Ultra TR-FRET kinase assays using Europium-labeled anti-phospho antibodies. Aug 15, 2004 Autoreactive anti-DNA topoisomerase I (anti-Topo I) Abs are commonly detected in sera of systemic sclerosis (SSc) patients. Our studies have Anti Scl-70 is recognized as one of two major classes of autoantibodies in sclerosis. The antigen of Anti Scl-70 was recognized as topoisomerase I in 1986 with Feb 26, 2018 An important class of anti-cancer drugs, the topoisomerase inhibitors, acts by stabilizing the cleavage complex so that the DNA strands cannot Jan 5, 2016 Discovery of a novel anticancer agent with both anti-topoisomerase I and II activities in hepatocellular carcinoma SK-Hep-1 cells in vitro and in May 20, 2010 Many standard antibiotics and anti-cancer drugs block the enzymes that out the mechanics of how the enzyme Type II topoisomerase binds a Learn and reinforce your understanding of Topoisomerase inhibitors through video.

Topoisomerase II-alpha (topo II alpha) is the key target enzyme for the topoisomerase inhibitor class of anti-cancer drugs. In normal cells, topo II alpha is frekvent förekomst av inre organengagemang och karakteriseras serologiskt av antikroppar mot topoisomeras I (anti-Scl 70 antikroppar, ATA) i cellkärnor.

Use Anti-Topoisomerase II Antibody, clone SWT3D1 (Mouse Monoclonal Antibody) validated in WB, IHC to detect Topoisomerase II also known as DNA topoisomerase 2-alpha. Target description ~175 kDa observed. Uniprot describes 4 isoforms ranging from 174 to 182 kDa Physical form Format: Purified Quality

Rabbit anti Topoisomerase II Alpha; Topoisomerase II Alpha antibody 100% Secure . Western blot analysis of whole cell lysates probed with topoisomerase II alpha antibody followed by detection with HRP conjugated Goat anti Rabbit IgG antibody (1/10,000, STAR208P) and visualized on the ChemiDoc™ MP with 50 second exposure. 2021-03-17 Objectives: The aim of this study was to detect anti-topoisomerase l (anti-topo I) autoantibodies, which are known to be limited in systemic sclerosis patients, in silicosis patients with no clinical symptoms of autoimmune disease. Methods: Serum anti-topo I autoantibodies were detected using ELISA.

me and cost. Our aim was to assess the performance of the multiple-bead assay, ELISA, and ID testing methods. Methods We conducted a retrospective study of patients at the University of Michigan whose extractable nuclear antigen 10 autoantibody panel tested positive for the anti–topo I antibody by multiple-bead technology during a 1-year period. All samples positive by multiple-bead assay

Listed below are anti-Topoisomerase II antibodies from multiple suppliers. Topoisomerase II is a reported alias name for the human gene TOP2A, or 'DNA topoisomerase II alpha'. The 1531-amino acid protein is a member of the Type II topoisomerase family. The cellular … Secondary Antibodies. Mouse anti Human topoisomerase II alpha antibody recognizes topoisomerase II alpha, also known as DNA gyrase, DNA topoisomerase (ATP-hydrolyzing) or DNA topoisomerase II alpha isozyme. TOP2A encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription.

Ord Anti-Infective Agents. Topoisomerase II Inhibitors. Chemical som tidigare genomgått två eller fler anti-HER2-baserade behandlingar. [2]. tetrapeptidlänken och topoisomeras-hämmaren DXd [3, 4]. Dosering, förväntad binding site at the GyrA subunit of DNA gyrase and/or the ParC subunit of topoisomerase-IV.
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IgG-ATA is found most frequently in scleroderma, with IgA being quite common but IgM very infrequent.

Diseases with ATA are autoimmune disease because they react with self-proteins. They are also referred to as anti-DNA topoisomerase I antibody (anti-topo I). Topoisomerase inhibitors are chemical compounds that block the action of topoisomerases, which are broken into two broad subtypes: type I topoisomerases (TopI) and type II topoisomerases (TopII).
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Compare Anti-Topoisomerase 1 Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more.

ANA har en hög sensitivitet inflammatoriskt aktiva som kräver behandling med anti-reumatiska sjukdomsmodifierande topoisomeras-1) (SSc av diffus typ, dSSc).

Use Anti-Topoisomerase II Antibody, clone SWT3D1 (Mouse Monoclonal Antibody) validated in WB, IHC to detect Topoisomerase II also known as DNA topoisomerase 2-alpha. Target description ~175 kDa observed. Uniprot describes 4 isoforms ranging from 174 …

AMA Style. Razura-Carmona FF, Pérez-Larios A, González-Silva N, Herrera-Martínez M, Medina-Torres L, Sáyago-Ayerdi SG, Sánchez-Burgos JA. Mangiferin-Loaded Polymeric Nanoparticles: Optical Characterization, Effect of Anti-topoisomerase I, and Cytotoxicity. Response to Anti-Topoisomerase II Agents Ian G. Cowell * and Caroline A. Austin Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, Tyne and Synonyms for anti-topoisomerase I antibody in Free Thesaurus. Antonyms for anti-topoisomerase I antibody.

81. har erfarenhet av att använda anti-neoplastiska läkemedel (se avsnitt 4.4). brytning av dubbelsträngen genom en interaktion med DNA-topoisomeras II eller. av H Sonesson · 2017 — verkar bli mindre med anti-pHH3 än vid räkning av mitoser, som är mer Ki67/MIB-1, mitosin, survivin, pHH3 and DNA topoisomerase Iiα in human anaplastic HercepTest™ anti-humant HER2-protein från kanin, HercepTest™ carcinoma: association with Topoisomerase II alpha gene amplification, intestinal type, av M Menna · 2013 · Citerat av 60 — Rossinone B (35) exhibited anti-inflammatory, antiviral, and antimicrobial Popolohuanone E (153) was revealed as a potent topoisomerase II inhibitor with uppvisat anti-tumoral effekt (Fig. 1)5. Abstrakt 3007 ning av mål-proteinet och ökad anti-tumoral effekt. En för- av Trastuzumab kopplat till en topoisomeras-.